IVIS
®
Lumina XRMS Series III Hardware Manual Chapter 10 | Troubleshooting 75
10.2 Photographic Image Is Unacceptable
Photograph imaging parameters are automatically controlled and generally produce a good quality
photo. If shiny objects are imaged, creating specular reflections, the automatic algorithm may get
confused and produce an underexposed image.
Also, refer to the Living Image Software
®
Manual for IVIS Lumina XRMS Series III for further details
on acquiring images.
10.3 Luminescent Image Is Unacceptable
Binning, F/Stop, and exposure time affect the appearance of a luminescent image. Please refer to the
Living Image Software Manual for IVIS Lumina XRMS Series III for instructions on setting binning,
exposure time, and F/Stop values.
In order to function properly and reduce camera noise, the CCD camera must be cooled to the demand
temperature before acquiring an image. If the camera is not cooled to the demand temperature,
imaging may result in false positive signals.
Table 10.2 Troubleshooting – Unacceptable Photographic Image
Problem Possible Cause Corrective Action
Image is streaked. Subject moved during the
exposure.
Check to see if the subject may have moved. If the subject is
not on the sample stage, it is probably on the floor of the
imaging chamber. If the subject has moved, locate and re-
anesthetize it. If gas anesthesia is being used, confirm that
the anesthesia is turned on and the flow rate is appropriate.
Image is blurry. Subject height is significantly
less than or greater than 1.5 cm.
The focus is set for a sample height of 1.5 cm. Significant
deviation from this height results in an out-of-focus
photograph.
Incorrect F/Stop setting. The F/Stop for photographs should be set to F/Stop 8 or F/
Stop 6. An F/Stop smaller than 8 reduces the depth of field in
the photograph.
A white spot appears in the
center of the field of view.
An excessively moist
environment in the imaging
chamber can result in
condensation on the CCD
window (Figure 10.2.)
Turn off the entire system and remove excess moisture in the
imaging chamber. Allow the chamber to thoroughly dry. If
the problem persists, contact PerkinElmer Technical Support
for assistance (see page 24).
Figure 10.2 Example of Condensation
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