Roche Cobas 4800 System User Manual

cobas® 4800
System
Systern
Manual
Glossary
Arnp4fïcation
- Saniple carrier
The
glossary explains
key terms
used
in this
documentation.
Amplification
The process
of producing
many
DNA
copies from
one original DNA
or
RNA target
region.
PCR
is a nucleic
acid amplification
technique.
Anneal
The biochemical
process
of hybridizing
or
binding
two segments
of
complementary
nucleic
acid.
Autoloader
The
hardware assembly
on the
instrument
that
enables
automatic
loading
of
carriers
placed
on the
loading
deck.
It consists
of a loading head
movable
in the
Y
direction,
which draws
the items into
the instrument
and
can read the
barcodes on
them.
Control unit
A personal
computer
that runs
the
Microsoft
Windows
operating
system
and the software.
Controls Reagent
formulated
to
produce known
resuits
that
are
processed
like samples.
The
software monitors
control
resuits. Each
run requires
a positive
and a
negative
control.
Ct value
The amplification/detection cycle where
the
growth curve
crosses a specific
fluorescence
level and
the
growth
rate can
be observed.
Deepweli
plate
Plate used
for extraction
on the
instrument.
Denaturation
The process
of separating
double
stranded
DNA
into single strands
by
breaking
the
hydrogen
bonds.
Detection
Obtaining
measurements
to determine
whether
a
sample
is reactive for
the
target
analyte.
Fluorescence
measurements
are
made at
selected
temperatures
and times
during the
amplification
process.
When
the run
is complete
data are
analyzed to
determine
the
presence
of
the amplified
products
from the
target
and
internal control
nucleic acid
sequences.
DNA
Deoxyribonucleic
Acid
(DNA)
is the genetic
material that
is passed from
parent
to progeny
and
propagates the
characteristics
of the
species in
the
form
of
genes it contains
and the proteins
for which it
codes.
DNA
contains the
following four
nucleotides:
dATP,
dCTP,
dTTP, and dGTP.
Instrument
deck
The work surface
of
the
instrument,
where
pipetting takes
place. It is organized
as a
left-to
right
series
of tracks, into
which the
autoloader
draws
carriers.
LAN Local
Area Network.
The
system control
unit is
con nected to
the instrument
and
the analyzer.
Roche Diagnostics
115
Laboratnry
information
system.
A
computerized
system
for entering,
managing
and reporting
laboratory
information.
Information
includes
but
is not
limited
to
patient
demographics,
test orders,
and
test results.
Loading
deck
The external shelfat
the front of
the
instrument.
It provides
a surface onto
which carriers
can
be
placed before
the
autoloader
draws them
into the
instrument.
Magnetic
glass
particles
Used in combination
with
other
reagents to
bind
nucleic
acids,
allowing
separation
of
impurities
during
washing.
MGP
see Magnetic
Glass Particles.
Microweil
plate
Plate used
for amplification
and
detection.
The 96-well
plate is
barcoded
and has to
be
sealed
with a special
sealing film before
inserting into
the
analyzer.
PCR
Polymerase Chain
Reaction.
The
in vitro process
used
to
amplify
short specific
target
nucleic
acid
sequences.
PCR is performed
by cycling
the
temperature
of
the amplification
mixture
according
to a set
profile.
The
profile
generally consists
of denaturation
and
annealing.
Pipetting
channel A
hardware assembly
mounted
on
the
pipetting
arm. It can pick
up and
eject
tips, aspirate
and
dispense liquid,
and detect
liquid level
in tubes.
There
are 8 pipetting
channels
working in parallel.
Pipetting
tip
A disposable
tip for pipetting.
The tips
used
by
the instrument
are capable
of
transferring
up to
1000 microliters
of
liquid per pipetting
operation. The
tip includes
a buik-in filter
that prevents
the
possibility
of
liquid
inside
the tip entering
the
pipetting
channel
hardware.
Plate carrier
Carrier for
deepwell
plate and
microweil
plate
on the instrument.
Reagent reservoir
The
reagent reservoirs
hold the
reagents
used for sample
preparation.
The reagent
reservoirs
are barcoded
and are filled
manually
by
the
operator
(scan-scan-pour-place
principle)
for each run.
Reagent
reservoirs
are available
in two sizes:
200 mL and
50
mL.
Sample
carrier
Used to bad
unprocessed
samples
onto
and to unload
processed
samples from
the
instrument.
System Manual
. Version 1.0
145

Roche Cobas 4800 System Specifications

General

Brand	Roche
Model	Cobas 4800 System
Category	Analytical Instruments
Language	English