35
13. For conformity evaluation calculate the random
error of the measurement:
as standard deviation s = ∑(V
i
–
⊽)
2
n –1
n = number of measurement (10)
or as coefficient of variation CV = 100s/
⊽
14. Compare the systematic error (inaccuracy) and
random error (imprecision) to the performance
specification values in chapter 14., or to the
specifications of your own laboratory.
NOTE: Systematic error is the difference between
the dispensed volume and the selected test volume.
Random error is the scatter of the dispensed volumes
around the mean of the dispensed volume. (ISO 8655-
1.)
NOTE: Sartorius specifications are achieved in
strictly controlled conditions (ISO 8655-6). Users
should establish acceptable maximum permissible
errors based on the field of use and the accuracy
requirements placed on the pipette (ISO 8655-1).
Z-values (µl/mg):
Temp. (°C) Air Pressure (kPa)
95 100 101.3 105
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0028
1.0029
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0037
NOTE:
This method is based on ISO 8655.
12.1. Recalibration
The eLINE® electronic pipette’s calibration has been
factory checked and certified at 22°C using grade 3
distilled water according to ISO 8655. The pipette’s
specifications are guaranteed only with genuine
Sartorius tips.
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