Thermo Scientific UltiMate - Page 36

Test Procedures
Page 36 of 60 M1393R0 OQ/PQ Operating Instructions
The programs used for the gradient accuracy tests are listed in TABLE 4-4 and TABLE 4-5.
TABLE 4-4 Program for the Gradient Accuracy Test on a NAN Configuration
Time
[min]
%A %B Wavelength
[nm]
0.00 100 0 254
10.00 100 0 “
10.01 95 5 “
20.00 95 5 “
20.01 50 50 “
30.00 50 50 “
30.01 5 95 “
40.00 5 95 “
40.01 0 100 “
50.00 0 100 “
50.01 100 0 “
80.00 100 0 “
TABLE 4-5 Program for the Gradient Accuracy Test on a CAP Configuration
Time
[min]
%A %B Wavelength
[nm]
0.00 100 0 254
5.00 100 0 “
5.01 95 5 “
10.00 95 5 “
10.01 50 50 “
15.00 50 50 “
15.01 5 95 “
20.00 5 95 “
20.01 0 100 “
25.00 0 100 “
25.01 100 0 “
50.00 100 0 “
Experimental conditions for the step gradient test for nano- and capillary LC systems are
listed in TABLE 4-6.
TABLE 4-6 Experimental Conditions for the Step Gradient Test.
Parameter Nano Configuration Capillary Configuration
Solvents A: HPLC – Water
B: HPLC – Water with Acetone
C: 100% ACN + 0.1% FA (a)
D: 100% Water + 0.1% FA
Acetone concentration of
Solvent B
0.8% 0.3%
Detection wavelength 254 nm 254 nm
Run time 80 min 50 min
Flow rate
0.4 µl/min 4.0 µl/min
Note: a) The acetonitrile is used to perform a wash step during the
FAMOS test procedure 4.8.