Thermo BioMate 3 series User Manual

completes the measurement, it returns to its
previous cell position.
4. Press Measure Sample to start the
measurement. If your instrument is equipped
with a 6-Position Cell Holder, press the cell
position buttons on the keypad to re-position
the cell holder and measure the rest of the
unknowns manually.
5. When the instrument has measured all the
unknowns, it displays the absorbance and
concentration.
Oligo Calculator
The oligonucleotide calculator determines the
following data for a base sequence that you enter:
• Number of bases
• Percent GC content
• Molecular weight
• Absorptivity (ε)
• Conversion factor to convert nucleotide
absorbance to concentration
• Tm for oligos of up to 20 bases
• Tm for oligos of up to 40 bases for DNA-DNA,
DNA-RNA and RNA-RNA hybrids
Refer to Appendix B for a description of the
parameters and Appendix C for the default values.
To get started, move the arrow keys on the
BioMate Tests screen to highlight Oligo
Calculator and press ENTER. The Oligos screen
appears.
Using the oligo calculator
1. With the Oligos screen displayed, press Base
Sequence. The Base Sequence identification
screen appears.
2. Use the arrow keys to select the appropriate
character for the base you want to enter. Press
Add Base to add the base to the sequence.
3. When the base sequence is correct, press
Accept Sequence to accept it. The instrument
calculates and displays the results
4. To determine the theoretical Tm of the
sequence, press Tm Calc. The Tm calculation
screen appears.
5. Enter the % formamide and % mismatch (if
known) that will be used to calculate the Tm.
The calculated Tm values are shown on the
screen.
Using “Biotests” Software
Thermo Electron Corporation 2-12

Thermo BioMate 3 series Specifications

General

Brand	Thermo
Model	BioMate 3 series
Category	Measuring Instruments
Language	English