• When you use a factor, you need to specify the
factor and the concentration units.
• When you use a standard, you need to specify
the concentration of the standard and measure
its absorbance.
When you use the Basic Concentration program,
you can perform these tasks:
• Set the wavelength
• Measure a blank
• Measure a standard OR enter a factor
• Measure unknowns
The steps for taking measurements in the two
modes are similar - the only difference will be
whether you measure a standard or enter a factor.
Setting the wavelength & mode
1. Press Set nm or any number key to set the
wavelength.
2. Enter the wavelength where you want the
measurements taken, then press Set nm again.
3. Press Change Mode until the appropriate
measurement mode (Concentration with
Standard or Concentration with Factor)
appears.
Measuring a blank
1. Place the blank in the cell holder. If your
instrument has a 6-Position Cell Holder, be
sure to place the blank in the B position.
2. If you want to enter a concentration for the
blank, press a number key and enter the
concentration in the Entry field.
3. Press Measure Blank to measure the blank.
When the instrument is finished measuring the
absorbance of the blank, the message
disappears.
Measuring a standard
1. If necessary, press Change Mode to switch to
the Concentration with Standard mode.
2. If your instrument is equipped with the
6-Position Cell Holder, place the standard in
one of the cell positions and press the
corresponding cell position key on the keypad.
If your instrument is equipped with the Single
Cell Holder Platform, remove the blank and
place the standard in the cell holder.
3. Press Units/Standard to set the units and
measure the standard.
4. Enter Conc, use the number keys to enter the
concentration value of the standard and then
press ENTER.
5. Press Select Units, use the arrow keys to
highlight the entry in the Units field and then
press ENTER to select the units for the
concentration.
6. Press Measure Standard to measure the
standard. When the instrument is finished
measuring the absorbance of the standard, it
displays the absorbance and calculated factor.
Using “General Tests” Software
3-7 BioMate 3 Operator’s Manual
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