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Thermo NanoDrop One User Manual

Thermo NanoDrop One
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NanoDrop Micro-UV/Vis Spectrophotometers
NanoDrop One
User Guide
269-309101 Revision B July 2016

Table of Contents

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Thermo NanoDrop One Specifications

General IconGeneral
Wavelength Range190-850 nm
Pathlength1 mm and 0.2 mm (Auto-ranging)
Detection Limit2 ng/µL (dsDNA)
Light SourceXenon flash lamp
Maximum Concentration15, 000 ng/µL (dsDNA)
Detector2048-element linear silicon CCD array
Measurement Time< 5 seconds
Weight2.0 kg (4.4 lbs)
Nucleic Acid QuantitationYes
Protein QuantitationYes
Display7-inch capacitive touch screen
Sample Volume1-2 μL
Absorbance Precision±0.002 A
Operating Voltage100 to 240 V AC
Dimensions (W x D x H)20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
SoftwareThermo Scientific™ software
ConnectivityUSB, Ethernet, Wi-Fi
Operating Temperature15 - 35°C
Dimensions20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Absorbance Range0 - 550 A (10 mm equivalent)

Summary

About the NanoDrop One Spectrophotometer

Instrument Models and Features

Details the available NanoDrop One models and their key specifications.

Register Your Instrument

Instructions for registering the instrument to receive software updates and notifications.

Update Software

Guide for downloading and installing the latest NanoDrop One software for the instrument.

Applications

Detection Limits for All Applications

Provides lower and upper detection limits and typical reproducibility for various sample types.

Measure dsDNA, ssDNA or RNA

Quantifies purified double-stranded or single-stranded DNA/RNA, reporting concentration and purity ratios.

Measure Protein A280

Measures protein concentration using absorbance at 280 nm, useful for proteins with tryptophan or tyrosine.

Measure Protein BCA

Determines total protein concentration using bicinchoninic acid colorimetric detection.

Measure OD600

Monitors microbial cell culture growth by measuring optical density at 600 nm.

Measure UV-Vis

Functions as a conventional spectrophotometer, displaying absorbance across UV and visible spectrum.

Measure Kinetics

Performs time-based kinetic measurements using cuvettes on NanoDrop OneC instruments.

Learning Center

Micro-Volume Sampling—How it Works

Explains the surface tension-based sample retention system for micro-volume measurements.

Basic Instrument Operations

Overview of fundamental operations like Home Screen, Measurement Screens, Data Viewer, and General Operations.

NanoDrop One Viewer Software

Details the companion software for managing, viewing, and analyzing data acquired with the instrument.

Maintaining Your Instrument

Maintenance Schedule

Outlines daily, periodic, and bi-annual maintenance tasks to ensure instrument performance.

Maintaining the Pedestals

Provides procedures for cleaning and reconditioning the instrument pedestals for optimal measurements.

Instrument Diagnostics

Describes performance and quality checks to verify instrument operation every six months.

Safety and Operating Precautions

Safety Information

Presents safety symbols and their meanings to ensure safe operation and handling.

Operating Precautions

Lists essential precautions to prevent instrument damage and ensure safe usage.

Contact Technical Support

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