Thermo NanoDrop One User Manual

4
Maintaining Your Instrument
Instrument Diagnostics
Thermo Scientific NanoDrop One User Guide 291
Instrument Diagnostics
Every 6 months, run the following performance and quality checks to verify instrument
operation.
Intensity Check
Run Intensity Check every 6 months to verify operation of the instrument’s internal
components. The test measures the intensity of light from the xenon source through the
instrument to verify that throughput, wavelength accuracy, and bias are within specifications.
If the instrument has a cuvette holder (NanoDrop One
C
model only), the test is automatically
repeated using the cuvette optical path.
Supplies needed
• lint-free laboratory wipes
To run intensity check
1. Lift the instrument arm and clean the upper and lower pedestal with a new laboratory
wipe.
2. For the NanoDrop One
C
model instrument, remove any cuvette from the cuvette holder.
3. Lower the arm.
4. From the instrument home screen, tap (Diagnostics) and then tap
Intensity Check.
Intensity Check Performance Verification
Pedestal Image Check

Thermo NanoDrop One Specifications

General

Wavelength Range	190-850 nm
Pathlength	1 mm and 0.2 mm (Auto-ranging)
Detection Limit	2 ng/µL (dsDNA)
Light Source	Xenon flash lamp
Maximum Concentration	15, 000 ng/µL (dsDNA)
Detector	2048-element linear silicon CCD array
Measurement Time	< 5 seconds
Weight	2.0 kg (4.4 lbs)
Nucleic Acid Quantitation	Yes
Protein Quantitation	Yes
Display	7-inch capacitive touch screen
Sample Volume	1-2 μL
Absorbance Precision	±0.002 A
Operating Voltage	100 to 240 V AC
Dimensions (W x D x H)	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Software	Thermo Scientific™ software
Connectivity	USB, Ethernet, Wi-Fi
Operating Temperature	15 - 35°C
Dimensions	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Absorbance Range	0 - 550 A (10 mm equivalent)