4
Maintaining Your Instrument
Instrument Diagnostics
Thermo Scientific NanoDrop One User Guide 293
To interpret intensity check results
If one of these indicators:
•UV
• Visible
•Bias
has an adjacent yellow triangle instead of the green check marks shown above, clean the
pedestals with deionized water and then repeat the Intensity Check.
If a yellow triangle appears next to the Bias indicator, make sure the room is within the
temperature specifications for the instrument.
If the Intensity Check fails again, contact us.
Related Topics
• Performance Verification
• Pedestal Image Check
Performance Verification
Run Performance Verification every 6 months to confirm pathlength accuracy is within
specifications.
Supplies needed
• lint-free laboratory wipes
•deionized water (DIH
2
O)
• calibrated precision pipettor (0–2 μL)
• PV-1 performance verification solution (liquid photometric standard available only from
us or a local distributor)
• laboratory gloves
Note The PV-1 solution comes in a single-use ampoule. Before you open the ampoule,
shake it vigorously and then allow the liquid to collect in the bottom portion of the
ampoule. After the ampoule is opened, its contents must be used within one hour. Pipette
directly from the ampoule; do not transfer the solution.
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