Thermo NanoDrop One User Manual

Thermo Scientific NanoDrop One User Guide iii
C
Chapter 1 About the NanoDrop One Spectrophotometer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Instrument Models and Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Optional Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Register Your Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Update Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 2 Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Detection Limits for All Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Measure dsDNA, ssDNA or RNA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Measure dsDNA, ssDNA or RNA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Nucleic Acid Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Setting for Nucleic Acid Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Calculations for Nucleic Acid Measurements. . . . . . . . . . . . . . . . . . . . . . . . . 18
Measure Microarray. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Measure Microarray Samples. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Microarray Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Settings for Microarray Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Calculations for Microarray Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Measure using a Custom Factor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Measure Nucleic Acid using a Custom Factor . . . . . . . . . . . . . . . . . . . . . . . . 35
Custom Factor Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Settings for Nucleic Acid Measurements using a Custom Factor . . . . . . . . . . 39
Detection Limits for Nucleic Acid Measurements using a Custom
Factor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Measure Oligo DNA or Oligo RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Measure Oligo DNA or Oligo RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Oligo Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Settings for Oligo DNA and Oligo RNA Measurements . . . . . . . . . . . . . . . . 47
Detection Limits for Oligo DNA and Oligo RNA Measurements . . . . . . . . . 48
Calculations for Oligo DNA and Oligo RNA Measurements . . . . . . . . . . . . 49
Contents

Thermo NanoDrop One Specifications

General

Wavelength Range	190-850 nm
Pathlength	1 mm and 0.2 mm (Auto-ranging)
Detection Limit	2 ng/µL (dsDNA)
Light Source	Xenon flash lamp
Maximum Concentration	15, 000 ng/µL (dsDNA)
Detector	2048-element linear silicon CCD array
Measurement Time	< 5 seconds
Weight	2.0 kg (4.4 lbs)
Nucleic Acid Quantitation	Yes
Protein Quantitation	Yes
Display	7-inch capacitive touch screen
Sample Volume	1-2 μL
Absorbance Precision	±0.002 A
Operating Voltage	100 to 240 V AC
Dimensions (W x D x H)	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Software	Thermo Scientific™ software
Connectivity	USB, Ethernet, Wi-Fi
Operating Temperature	15 - 35°C
Dimensions	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Absorbance Range	0 - 550 A (10 mm equivalent)