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Thermo NanoDrop One - Default Chapter; Table of Contents

Thermo NanoDrop One
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Thermo Scientific NanoDrop One User Guide iii
C
Chapter 1 About the NanoDrop One Spectrophotometer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Instrument Models and Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Optional Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Register Your Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Update Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 2 Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Detection Limits for All Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Measure dsDNA, ssDNA or RNA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Measure dsDNA, ssDNA or RNA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Nucleic Acid Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Setting for Nucleic Acid Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Calculations for Nucleic Acid Measurements. . . . . . . . . . . . . . . . . . . . . . . . . 18
Measure Microarray. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Measure Microarray Samples. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Microarray Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Settings for Microarray Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Calculations for Microarray Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Measure using a Custom Factor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Measure Nucleic Acid using a Custom Factor . . . . . . . . . . . . . . . . . . . . . . . . 35
Custom Factor Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Settings for Nucleic Acid Measurements using a Custom Factor . . . . . . . . . . 39
Detection Limits for Nucleic Acid Measurements using a Custom
Factor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Measure Oligo DNA or Oligo RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Measure Oligo DNA or Oligo RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Oligo Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Settings for Oligo DNA and Oligo RNA Measurements . . . . . . . . . . . . . . . . 47
Detection Limits for Oligo DNA and Oligo RNA Measurements . . . . . . . . . 48
Calculations for Oligo DNA and Oligo RNA Measurements . . . . . . . . . . . . 49
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