Thermo NanoDrop One User Manual

Contents
iv NanoDrop One User Guide Thermo Scientific
Measure Protein A280. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Measure Protein Concentration at A280 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Protein A280 Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Settings for Protein A280 Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Detection Limits for Protein A280 Measurements. . . . . . . . . . . . . . . . . . . . . 64
Calculations for Protein A280 Measurements . . . . . . . . . . . . . . . . . . . . . . . . 65
Measure Proteins and Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Measure Labeled Protein Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Proteins & Labels Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Settings for Proteins and Labels Measurements . . . . . . . . . . . . . . . . . . . . . . . 74
Detection Limits for Proteins and Labels Measurements . . . . . . . . . . . . . . . . 76
Calculations for Proteins and Labels Measurements. . . . . . . . . . . . . . . . . . . . 77
Measure Protein A205. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Measure Protein Concentration at A205 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Protein A205 Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Settings for Protein A205 Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Calculations for Protein A205 Measurements . . . . . . . . . . . . . . . . . . . . . . . . 85
Measure Protein BCA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Measure Total Protein Concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Protein BCA Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
Settings for Protein BCA Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Measure Protein Bradford . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Measure Total Protein Concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Protein Bradford Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Settings for Protein Bradford Measurements . . . . . . . . . . . . . . . . . . . . . . . . 109
Measure Protein Lowry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Measure Total Protein Concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Protein Lowry Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Settings for Protein Lowry Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Measure Protein Pierce 660. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Measure Total Protein Concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Protein Pierce 660 Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Settings for Protein Pierce 660 Measurements . . . . . . . . . . . . . . . . . . . . . . . 127
Measure OD600 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
Measure OD600 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
OD600 Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Settings for OD600 Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
Calculations for OD600 Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Measure Custom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
Measure using a Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
Delete Custom Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
Custom Method Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144

Thermo NanoDrop One Specifications

General

Wavelength Range	190-850 nm
Pathlength	1 mm and 0.2 mm (Auto-ranging)
Detection Limit	2 ng/µL (dsDNA)
Light Source	Xenon flash lamp
Maximum Concentration	15, 000 ng/µL (dsDNA)
Detector	2048-element linear silicon CCD array
Measurement Time	< 5 seconds
Weight	2.0 kg (4.4 lbs)
Nucleic Acid Quantitation	Yes
Protein Quantitation	Yes
Display	7-inch capacitive touch screen
Sample Volume	1-2 μL
Absorbance Precision	±0.002 A
Operating Voltage	100 to 240 V AC
Dimensions (W x D x H)	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Software	Thermo Scientific™ software
Connectivity	USB, Ethernet, Wi-Fi
Operating Temperature	15 - 35°C
Dimensions	20 cm x 25 cm x 15 cm (8 in. x 10 in. x 6 in.)
Absorbance Range	0 - 550 A (10 mm equivalent)