Thermo Scientific NanoDrop One User Guide 41
Measure Oligo DNA or Oligo RNA
Measure Oligo DNA or Oligo RNA
Use the Oligo DNA and Oligo RNA applications to quantify oligonucleotides that absorb at
260 nm. Molar extinction coefficients are calculated automatically based on the user-defined
base sequence of the sample. These applications report nucleic acid concentration and two
absorbance ratios (A260/A280 and A260/A230). A single-point baseline correction can also
be used.
Measures the concentration of
purified ssDNA or RNA
oligonucleotides that absorb at
260 nm.
Measure Oligo DNA or RNA
Reported Results
Settings
Detection Limits
Calculations
Note If the oligonucleotide has been modified, for example with a fluorophore dye, check
with the oligo manufacturer to determine if the modification contributes absorbance at
260 nm. If it does, we recommend using the Microarray application to quantify nucleic
acid concentration. The Microarray application includes a correction to remove any
absorbance contribution due to the dye from the oligo quantification result.
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