Measure Oligo DNA or Oligo RNA
Thermo Scientific NanoDrop One User Guide 47
Settings for Oligo DNA and Oligo RNA Measurements
The Oligo setup screen appears after you select the Oligo DNA or Oligo RNA application
from the Nucleic Acids tab on the Home screen. To show the Oligo settings from the
Oligo DNA or Oligo RNA measurement screen, tap > Oligo DNA Setup (or
Oligo RNA Setup).
Setting Available Options Description
Oligo Base
Sequence
for DNA: Use the
G, A, T and C keys to
specify the DNA base
sequence
for RNA: Use the
G, A, U and C keys to
specify the RNA base
sequence
Specify your DNA or RNA base sequence by tapping the corresponding keys:
Each time a base is added to the sequence, the software calculates the following:
• Factor. Constant used to calculate oligonucleotide concentration in modified
Beer’s Law equation. Based on extinction coefficient and pathlength:
f = 1/(
260
* b)
where:
f= factor
= molar extinction coefficient at 260 nm in ng-cm/μL
b = sample pathlength in cm (0.1 cm for nucleic acids measured with the
NanoDrop One instrument)
Add guanine
Add adenine
Add thymine (DNA)
or uracil (RNA)
Add cytosine
Remove most
recent base
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