Deleting wavelengths
1. If you want to delete a wavelength, use the
arrow keys to highlight the appropriate
wavelength.
2. When the appropriate wavelength is
highlighted, press Delete nm to confirm that
you want to delete the wavelength.
Running the test
1. With the Wavelength Accuracy - Standards
screen displayed, make sure that the
wavelengths and tolerances are set correctly.
2. Press Start Test to run the test. The results of
the test appear on the screen, indicating pass
or fail for each wavelength.
If the test fails, follow these guidelines:
• Repeat the test several times to verify that the
test is failing consistently.
• Make sure the target value you entered for the
calibrated wavelength is the same as the value
printed on the SPECTRONIC Standards
certificate.
• Make sure the tolerance value you entered for
the calibrated wavelength is the same as the
value given in the SPECTRONIC Standards
User's Manual.
Photometric Accuracy
This test measures the absorbance (or
%transmittance) of a set of standards and
compares the results with specified tolerances. The
absorbances and tolerances are preset, but you
should change them to the values on the report of
calibration included with your standards.
Note: You can display the tolerances for this test
in either absorbance or %transmittance.
1. With the Performance Validation screen
displayed, use the arrow keys to highlight
Photometric Accuracy.
2. Press ENTER. The Photometric Accuracy
screen appears.
Selecting the mode
1. To switch between absorbance and
%transmittance, press Change to %T (or
Change to ABS) until the appropriate mode
appears.
Adding standards
You will need to set three values whenever you
add a standard - the wavelength, the absorbance
(or %transmittance) and the tolerance value.
1. If you need to add a standard, press Add Std
and enter the wavelength value in the Entry
field.
2. When the wavelength value is correct, press
Add Std again to add the wavelength to the
list. The screen changes to prompt you to enter
the absorbance (or %transmittance) for the
wavelength you just entered.
3. Enter enter the tolerance value in the Entry
field.
4. Press Start Test or Press ESC to save test.
Using the Performance Validation Program
4-3 BioMate 3 Operator’s Manual