Waters 2998 - Page 116

C-16 Solvent Considerations
Acids and bases
Acetic acid,
1%
2.61 2.63 2.61 2.43 2.17 0.87 0.14 0.01 <0.01 —
Hydro-
chloric acid,
0.1%
0.110.02<0.01———————
Phosphoric
acid, 0.1%
<0.01—————————
Trifluoro-
acetic acid
1.20 0.78 0.54 0.34 0.22 0.06 <0.02 <0.01 — —
Diammo-
nium
phosphate,
50 mM
1.850.670.150.02<0.01—————
Triethy-
lamine, 1%
2.33 2.42 2.50 2.45 2.37 1.96 0.50 0.12 0.04 <0.01
Buffers and Salts
Ammonium
acetate,
10 mM
1.88 0.94 0.53 0.29 0.15 0.02 <0.01 — — —
Ammonium
bicarbonate,
10 mM
0.410.100.01<0.01——————
EDTA,
disodium,
1 mM
0.11 0.07 0.06 0.04 0.03 0.03 0.02 0.02 0.02 0.02
HEPES,
10 mM,
pH 7.6
2.45 2.50 2.37 2.08 1.50 0.29 0.03 <0.01 — —
MES,
10 mM,
pH 6.0
2.42 2.38 1.89 0.90 0.45 0.06 <0.01 — — —
Mobile phase absorbance measured against air or water (Continued)
Absorbance at specified wavelength (nm)
200 205 210 215 220 230 240 250 260 280