Computing absorbance data points 1-11
Resolution comparison for anthracene
Averaging chromatographic sampling rate
Sample rate is the number of data points acquired per second. The number of
times a given pixel is read during the sample rate interval depends on its
exposure time. For example, if exposure time is 25 msec and sample rate is 20
Hz, then exposures per sample is
3.20
3.30 3.40 3.50 3.60 3.70 3.80 3.90 4.00 4.10
4.20
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
0.50
0.55
Absorbance
252 nm at 1.2 nm, height 0.53
252 nm at 6.0 nm, height 0.45
252 nm at 12.0 nm, height 0.35
20 samples/second = = 2 exposures/sample
(1000 msec/second)
(20 samples/second)(25 msec/exposure)