OPERATION
Axio Observer Illumination and contrast techniques ZEISS
12/2016 431004-7244-001 153
5.12.4 Setting up transmitted light PlasDIC contrast
5.12.4.1 General operating principle
PlasDIC is an innovative transmitted light interference contrast technique that provides a relief-like image
and is particularly useful for thicker specimens. The contrast can be varied.
Microtiter plate wells can be contrasted right up to the edge.
Culture vessels with a glass bottom are not required, but are also suitable.
5.12.4.2 Instrument equipment
Objectives:
− LD A-Plan 10x to 63x (4212xx-xxxx-xxx)
− LD Plan-Neofluar 20x/0.4 Corr M27
− LD Plan-Neofluar 40x/0.6 Corr M27
− LD Plan-Neofluar 63x/0.75 Corr M27
At least one of these objectives must be available.
Condensers:
− LD condenser 0.35 H, Ph0, Ph1, Ph2, DIC, DIC; 6-position (424241-0000-000)
− LD condenser 0.55 H, Ph1, Ph2, Ph3, DIC, DIC; 6-position (424242-0000-000)
− LD condenser 0.55 H, Ph1, Ph2, Ph3, DIC, DIC; 6-position (424244-0000-000)
with
− Slit aperture 3.5 mm PlasDIC (000000-1246-773)
or
− LD condenser 0.35 H, Ph PlasDIC DIC iHMC (424241-9010-000)
with
− Slit aperture 3.5 mm PlasDIC for condenser (10x-40x) (426717-9110-000)
− Slit aperture 5 mm PlasDIC for condenser (40x/63x) (426717-9120-000)
Swivel the slit aperture for PlasDIC, which is fitted into the condenser, into the beam path. The
size of the slit aperture to be used (for PlasDIC) depends on the objective used.
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