1.0 Product Description
1.01 How the 3M™ Clean-Trace™ NGi Luminometer works to Measure Contamination
1.02 ATP and Surface Hygiene Testing
1.03 ATP and Water Testing
1.01 How the 3M™ Clean-Trace™ NGi Luminometer works to Measure
Contamination
The 3M™ Clean-Trace™ NGi Luminometer is a luminometer used in conjunction with 3M reagent kits to measure
levels of contamination on surfaces and in water samples. The technology used is Adenosine Triphosphate (ATP)
bioluminescence. ATP is a substance present in all animal and vegetable matter, including most food debris,
bacteria, fungi and other micro-organisms. Measurement of ATP is performed using an enzyme reaction which
occurs naturally in the tail of fireflies.
Luciferin/Luciferase (firefly reagent) + ATP = Light
The light emitted is in proportion to the amount of ATP. The intensity of the light emitted from a sample is
measured by the 3M™ Clean-Trace™ NGi Luminometer and is displayed in Relative Light Units (RLU).
1.02 ATP and Surface Hygiene Testing.
The 3M™ Clean-Trace™ NGi Luminometer, together with the appropriate test kit, is an effective method for
monitoring the hygiene status of surfaces. ATP from both micro-organisms and product residues are detected.
Measurement of "total ATP" provides an important insight to the overall cleanliness. Product residues remaining
on surfaces after cleaning will be a source of nutrients for any remaining micro-organisms and may also protect
the micro-organisms from disinfectant action. Because of the fast results, use of the 3M™ Clean-Trace™ NGi
Luminometer allows immediate remedial action to be taken if an unacceptable result is obtained. Surfaces can be
re-cleaned before use, ensuring good sanitation and contamination control.
The test kit instructions should be read carefully before use.
1.03 ATP and Water Testing.
Test kits are available for the assessment of contamination in liquid samples. Measurement of the total ATP in
final rinse water samples is an indicator of the effectiveness of Clean in Place (CIP) processes. The total ATP in
process and waste water, water tanks and cooling towers can be used to determine and monitor the efficiency of
the biocide treatment program.
Measurement of free or non microbial ATP can be performed in addition to total ATP for further information on the
nature of the contamination.
The test kit instructions should be read carefully before use.
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