1260 Infinity Binary LC - System User Guide 115
Appendix
6
Setting Up a Method using Edit Entire Method
Autosampler tab
Figure 25 Setup Method screen – High Performance Autosampler tab
•Injection Mode
• Injection volume sets the volume to be injected (example 1 µl),
• Standard injection indicates that no external needle wash is done,
• Injection with needle wash is used to reduce potential carryover. This is
the recommended option and is configured in the next entry.
• Needle Wash – if selected above.
• Mode determines how the outside of the needle will be rinsed: either
actively in the Flush Port or by dipping into a specified Wash Vial.
• Time in seconds the peristaltic pump connected to the flush port will
pump the rinse solvent. It then pumps for a further 15 s to clear the
flush port.
• Location determines which vial or well plate will be used if Wash Vial
was selected.
Vials should not have a septum i.e., they should be open to avoid transfer of carryover
material on the septum.
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