34 1260 Infinity Binary LC - System User Guide
2 Introduction
Benefits of small particle size columns
Benefits of small particle size columns
Faster Chromatography
There are several advantages of having shorter run times. High
Throughput labs now have higher capacity and can analyze more samples
in less time. More samples in less time also means lower costs. For
example, by reducing the analysis time from 20 min per sample to 5 min,
the cost for 700 samples is reduced by 79 % (Table 3 on page 34).
The Agilent cost savings calculator provides an easy way to calculate the
cost savings by switching from conventional HPLC to UHPLC using 1.8 µm
particle size columns. This calculator is available on the Agilent
Technologies web site along with a method translator calculator –
www.chem.agilent.com. The results are presented graphically and in
tabular form.
Shorter run times also deliver faster answers. This is important in process
control and rapid release testing. Instead of waiting hours to release a
single batch of a drug, all the system suitability, calibration and sample
analysis can now be done in less than an hour. Rapid answers are also
important for synthetic chemists using open access LC/MS systems for
compound confirmation and reaction control. Shorter run times can also
accelerate the method development process significantly.
Tabl e 3 Time and cost savings over 700 runs
Cycle time 20 min cycle time 5 min cycle time
Runs 700 700
Approx. costs/analysis
1
1
solvents = $ 27/l, disposal = $ 2/l, labor = $ 30/h
$ 10.58 $ 2.24
Approx. cost/700runs
1
$ 7400 $ 1570
Cost savings - $ 5830
Time
2
2
24 hours/day
10 days 2.5 days
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