28 1260 Infinity Binary LC - System User Guide
2 Introduction
Theory of Using Smaller Particles in Liquid Chromatography
Theory of Using Smaller Particles in Liquid Chromatography
Introduction
In 2003, Agilent introduced the first commercially available, totally porous
silica columns with 1.8 µm particles.
In combination with the Agilent 1260 Infinity Binary LC the sub- two
micron (1.8 µm) particle size columns can be used in pursuit of two main
objectives:
1 Faster Chromatography
Short columns with sub- two- micron particles offer the opportunity to
dramatically reduce analysis time by increasing the flow rate without
losing separation performance.
2 Higher Resolution
Long columns with sub- two- micron particles provide higher efficiency
and therefore higher resolution, which is required for the separation of
complex samples.
The pressure that is needed to drive solvent through a column containing
STM (sub- two micron) particles rises rapidly as flow rate is increased for
faster separations and very rapidly as the length of the column increases
for more resolution. Thus the acceptance of STM columns has been
synonymous with the development of UHPLC systems – that is HPLC
systems offering higher pressures than the 400 bar norm that was extant
since the early days of HPLC.
Today, Agilent offers the 1290 Infinity LC for highest UHPLC requirements
with pressures up to 1200 bar.
To Next Page
Loading...
Need help?
General
