1260 Infinity Binary LC - System User Guide 41
Optimization of the Agilent 1260 Infinity Binary LC
3
How to Configure the Optimum Delay Volume
Extra-Column Volume
Extra- column volume is a source of peak dispersion that will reduce the
resolution of the separation and so should be minimized. Smaller diameter
columns require proportionally smaller extra- column volumes to keep peak
dispersion at a minimum.
In a liquid chromatograph the extra- column volume will depend on the
connection tubing between the autosampler, column, and detector; and on
the volume of the flow cell in the detector. The extra- column volume is
minimized with the Agilent 1260 Infinity Binary LC due to the
narrow- bore (0.12 mm i.d.) tubing, the low- volume heat exchangers in the
column compartment and the Max- Light cartridge cell in the detector.
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