1260 Infinity Binary LC - System User Guide 93
Quick Start Guide
5
Data Acquisition in Method and Run Control View
4 Right- click the Thermostatted Column Compartment (TCC) area, and
select Method... in the context menu.
a In the Method page for the 1290 Infinity TCC, enter the following
parameters:
• Left Temperature 40 °C
• Right Temperature Combined
b Other parameters can remain at their default settings. Click OK to
exit the window.
The changes are sent to the TCC module.
5 Right- click the Diode- Array Detector area, and select Method... in the
context menu.
a In the Method page for the 1260 Infinity DAD, enter the following
parameters:
• Use Signal: Turn all signals except Signal A off by clearing the check
boxes.
• Signal A: 254 nm, bw 4 nm, ref 550 nm, bw 100 nm
• Peak width: 0.02
b In the Advanced section, set Spectrum Store to All.
c Other parameters can remain at their default settings. Click OK to
exit the window.
The changes are sent to the DAD module.
6 All the required module parameters have now been entered. Select
Method > Save Method As... and enter
ISO-1.M to save the method with a new name.
The ChemStation does not allow the method to be saved as DEF_LC.M so
that the default method template is not altered.
7 Allow the system to equilibrate for at least 10 min, and check that the
baseline in the Online Plot is stable before starting the analysis.
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