Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 21
Introduction to the Fluorescence Detector
1
Optical Unit
The radiation source is a xenon flash-lamp. The 3 s flash produces a
continuous spectrum of light from 200 nm to 900 nm. The light output
distribution can be expressed as a percentage in 100 nm intervals, see
Figure 8 on page 21. The lamp can be used for some 1000 hours depending on
the sensitivity requirements. You can economize during automatic operation
using keyboard setpoints, so the lamp flashes during your analysis only. The
lamp can be used until it no longer ignites, but the noise level may increase
with usage.
UV degradation, especially below 250 nm is significantly higher compared to
Visible wavelength range. Generally the "LAMP ON during run" - setting or
using "economy mode" will increase lamp life by a magnitude.
Figure 8 Lamp Energy Distribution (vendor data)
The radiation emitted by the lamp is dispersed and reflected by the excitation
monochromator grating onto the cell entrance slit.
The holographic concave grating is the main part of the monochromator,
dispersing and reflecting the incident light. The surface contains many minute
grooves, 1200 of them per millimeter. The grating carries a blaze to show
improved performance in the visible range.
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