24 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
1
Introduction to the Fluorescence Detector
Optical Unit
The flow cell is a solid fused silica body with a maximum back pressure of
20 bar. Excessive back pressure will result in destruction of the cell. Operating
the detector close to waste with low back pressure is recommended. A slit is
integrated to the body.
Figure 11 Cross-Section of Flow Cell
The luminescence from the sample in the flow cell is collected at right angles
to the incident light by a second lens, and passes through a second slit. Before
the luminescence reaches the emission monochromator, a cut-off filter
removes light below a certain wavelength, to reduce noise from 1
st
order
scatter and 2
nd
order stray light, see Figure 10 on page 23.
The selected wavelength of light is reflected onto the slit in the wall of the
photo-multiplier compartment of the optical unit. The bandwidth of the
emitted light is 20 nm.
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