Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 99
Optimizing the Detector
5
Method Development
Table 9 Conditions for Optimization of PNA analysis according to figures below
Column Vydac, 2.1 x 200 mm, PNA, 5 µm
Mobile phase A = water; B = acetonitrile (50 : 50)
Gradient 3 minutes, 60%
14 minutes, 90%
22 minutes, 100%
Flow rate 0.4 ml/min
Column temperature 18 °C
Injection volume 5 µl
FLD settings PMT 12,
response time 4 s,
step size 5 nm
This shows the
isofluorescence plot
of emission spectra
for 15 PNAs
(5 µg/ml) with a
fixed excitation
wavelength
(260 nm).
Figure 32 Optimization of the time-program for the emission wavelength
(PVSHFWUD
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