100 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
5
Optimizing the Detector
Method Development
Figure 33 Optimization of the time-program for the excitation wavelength
The obtained data are combined to setup the time-table for the excitation
wavelength for best limit of detection and selectivity. The optimized switching
events for this example are summarized in Table 10 on page 100.
This timetable gives the conditions for optimum detection based on the results
of two chromatographic runs.
Table 10 Timetable for the analysis of 15 polynuclear aromatic hydrocarbons
Time [min] Exitation Wavelength [nm] Emission Wavelength [nm]
0260 350
8.2 260 420
19.0 260 500
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