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2.7.4.1  Live Mode
Live Mode refers to a version of Run Till Abort in which each user frame will be the latest frame output by the 
sensor and will have the minimum amount of latency through the camera as possible.
LivemoderequirestheuseofSWTrigger.Inordertoensurethatframesarebueredintheon-cameramemory
for the shortest possible time, the next SW trigger must not be sent until the user frame has been transmitted 
from the camera. This ensures that the on-camera memory only stores a single user frame at a time and no 
additional latency builds up. 
NOTE: The frame rate achievable in Live Mode is dependent on the performance of the system that the 
camera is attached to.
2.7.5 Fast Exposure Switch 
During an acquisition the user can change the exposure time, within allowable limits. Once a new exposure 
value has been written, it will be applied to the next user frame after the current frame exposure has 
completed.Theexposuretimecanbechangedanynumberoftimesbeforetheacquisitionnishes
2.7.6 Frame Rate Control 
If Internal Trigger is being used, the camera will trigger the sensor at the fastest possible rate by default. The 
usercanreducethistriggerratebydeningaFrameRatethatislessthanthemaximumpossiblerate.Frame
Rate must be set before the acquisition starts.
Note: This is currently only applicable to ‘long exposures’.
2.7.7  LightScan PLUS
LightScan PLUS describes a functionality of the Zyla 4.2 PLUS camera that allows the end-user to have more 
control over the Rolling Shutter scanning mechanism that is inherent to CMOS sensors. This scanning mode can 
be readily adapted to niche optical modalities, such as Scanned Light Sheet microscopy or Line Scan Confocal 
Microscopy.
2.7.7.1 Multiple Readout Directions
The standard mode of operation in sCMOS cameras is to read out from the centre of the sensor out to the 
edge with two halves of the sensor exposing simultaneously. LightScan PLUS provides the user with a range 
ofdierentrollingshutterreadoutoptions.Firstly,therollingshuttercannowbescannedfromthetoptothe
bottom of the sensor or vice versa in one continuous sweep (Figure 34), adapting the sensor to single port 
readout. It should be acknowledged however that this functionality will halve the rolling shutter frame rate, 
resulting in a maximum full resolution frame rate of 50 fps.
Figure 34: LightScan PLUS enables the rolling shutter to scan the sensor from top to bottom or vice versa in one continuous sweep. This 
is also known as Single Port Readout.