Home
Applied Biosystems
Laboratory Equipment
7300
Applied Biosystems 7300 Getting Started Guide
5
of 1
of 1 rating
106 pages
Give review
Manual
Specs
To Next Page
To Next Page
Loading...
Intr
oduction and
Example
RQ
Experiment
Designing an
RQ
Experiment
Performing
Reverse
Tr
a
n
s
c
r
i
p
t
i
o
n
Generating Data
from RQ
Plates –
7300 or Standar
d
7500 System
Generating Data
from RQ
Plates –
7500 Fast System
Analyzing Da
ta in
an RQ Study
Primer Extended on mRNA
5
′
3
′
Reverse
Primer
5
′
Synthesis of 1st cDNA strand
cDNA
Oligo d(T) or random hexamer
3
′
5
′
cDNA
ST
ANDARD
S
TA
N
D
A
R
D
ST
ANDARD
FA
S
T
FA
S
T
FA
S
T
Applied Biosystems 7300/7500/7500 Fast
Real-Time
PCR System
Relative Quantification
Getting Started Guide
2
Table of Contents
Default Chapter
3
Relative Quantification Experiment Workflow
3
Table of Contents
5
How to Use this Guide
7
Preface
7
How to Obtain more Information
9
How to Obtain Support
10
Introduction
11
About Relative Quantification
12
About the 7300/7500/7500 Fast System
12
Chapter 1 Introduction
12
About RQ Experiments
13
Designing an RQ Experiment
17
Selecting the PCR Method
18
Chapter 2 Designing an RQ Experiment
18
Specifying the Components of an RQ Experiment
19
Selecting the Chemistry
21
Selecting One- or Two-Step RT-PCR
22
Choosing the Probes and Primers
24
Performing Reverse Transcription
27
Guidelines for Preparing RNA
28
Chapter 3 Performing Reverse Transcription
28
Converting Total RNA to Cdna
29
Chapter 4 Generating Data from RQ Plates - 7300 or Standard 7500 System
31
Before You Begin
32
Preparing the PCR Master MIX
32
Preparing the Reaction Plate
33
Creating a Relative Quantification (RQ) Plate Document
35
Specifying Thermal Cycling Conditions and Starting the Run
40
Analyzing and Viewing RQ Plate Data
43
Exporting RQ Plate Data
46
Chapter 5 Generating Data from RQ Plates - 7500 Fast System
47
Before You Begin
48
Preparing the PCR Master MIX
48
Preparing the Reaction Plate
50
Creating a Relative Quantification (RQ) Plate Document
53
Specifying Thermal Cycling Conditions and Starting the Run
58
Analyzing and Viewing RQ Plate Data
62
Exporting RQ Plate Data
65
Troubleshooting
66
Analyzing Data in an RQ Study
69
Creating an RQ Study Document
70
Chapter 6 Analyzing Data in an RQ Study
70
Configuring Analysis Settings
73
Adjusting the Baseline and Threshold
75
Analyzing and Viewing the Results of the RQ Study
80
Reanalyzing an RQ Study
84
Omitting Samples from a Study
85
Exporting RQ Study Data
88
Appendix A Creating Detectors
89
Appendix B Example RQ Experiment
92
References
100
Index
102
Other manuals for Applied Biosystems 7300
Installation And Maintenance Guide
174 pages
5
Based on 1 rating
Ask a question
Give review
Questions and Answers:
Need help?
Do you have a question about the Applied Biosystems 7300 and is the answer not in the manual?
Ask a question
Applied Biosystems 7300 Specifications
General
Brand
Applied Biosystems
Model
7300
Category
Laboratory Equipment
Language
English
Related product manuals
Applied Biosystems 7500
174 pages
Applied Biosystems 7900HT
384 pages
Applied Biosystems 7500 Fast
174 pages
Applied Biosystems ViiA 7 Real
22 pages
Applied Biosystems StepOne
184 pages
GeneAmp PCR System 9700
128 pages
QuantStudio 6 Flex
194 pages
Applied Biosystems 2720
122 pages
Applied Biosystems StepOnePlus
184 pages
Applied Biosystems QuantStudio 5
18 pages
To Next PageTo Next Page
Need help?
General
