Applied Biosystems 7300 - Introduction

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 1
Notes
1
Chapter 1
Introduction
Introduction
About the
7300/7500/7500 Fast
system
About Relative
Quantification
About
RQ Experiments
D
es
i
gn
i
ng
an R
Q
E
xper
i
men
t
Performin
g
R
everse
Transcriptio
n
P
r
i
m
e
r
E
xt
e
n
ded
o
n m
RNA
5
´
3
´
Revers
e
Prime
r
5
´
Sy
nthesis of 1st cDNA strand
cDNA
O
ligo d(T) or random hexame
r
3
´
5
´
cDNA
Performin
g
an R
Q
S
tud
y
OR
Generatin
g
Da
t
a
fr
om
RQ
Plate
s
S
tandard
STANDARD
TANDAR
G
eneratin
g
Da
t
a
fr
om
RQ
Plate
s
Fas
t
Seepage3
Seepage2
Seepage2