To Next PageTo Next PageWhy am I getting extremely high ?R or n R values with Applied Biosystems 7300?
Extremely high ?R or n R values with Applied Biosystems Laboratory Equipment can be due to a couple of reasons. First, ensure that ROX dye was selected as the passive reference when setting up the plate document; if not, select it. Also, check for evaporation by making sure that the reaction plate is sealed completely, especially around the edges.
Why is there high variability across the reaction plate with Applied Biosystems 7300?
High variability across the reaction plate when using Applied Biosystems Laboratory Equipment can be caused by a couple of factors. First, ensure that ROX dye was selected as the passive reference when setting up the plate document; if not, select it. Evaporation can also lead to variability; make sure that the reaction plate is sealed completely, especially around the edges, and use reagents that contain ROX passive reference dye.
What to do if I have low ?R or R values with Applied Biosystems 7300?
Low ?R or R values with Applied Biosystems Laboratory Equipment can occur due to a couple of reasons. If the extension time is too short, use the default thermal profile settings. Primer-dimer formation can also cause this; to ensure optimal results, run the reaction plate as soon as possible after completing the reaction setup. If you cannot run a reaction plate within 2 hours after completing the reaction setup, refrigerate or freeze the reaction plate until you can load and run it on the 7500 Fast instrument.
Why does the Applied Biosystems 7300 run take more than 40 minutes?
If a run with Applied Biosystems Laboratory Equipment takes more than 40 minutes, it is likely because the thermal cycler mode is set to Standard or 9600 Emulation. Ensure that the thermal cycler mode is set to Fast.
What causes high variability across replicates in Applied Biosystems Laboratory Equipment?
High variability across replicates in Applied Biosystems Laboratory Equipment can be caused by the reaction mix not being mixed well. To resolve this, mix the reaction mix gently by inversion, then centrifuge briefly before aliquoting to the reaction plate.
What to do if the Rn vs. Cycle plot is not displayed in Applied Biosystems 7300?
If the Rn vs. Cycle plot is not displayed when using Applied Biosystems Laboratory Equipment, it is likely because ROX dye was not selected as the passive reference when the plate document was set up. To fix this, select ROX dye as the passive reference when you set up the plate document.
General| Type | Real-Time PCR System |
|---|---|
| Detection Method | Fluorescence |
| Weight | Not found in official specification |
| Temperature Uniformity | ±0.5°C |
| Dimensions (W x D x H) | Not found in official specification |
| Detection Channels | 4 |
| Sample Capacity | 96-well |
| Temperature Range | 4°C to 100°C |
| Temperature Accuracy | ±0.25°C |
| Ramp Rate (heating) | 3.5°C/sec |
| Ramp Rate (cooling) | 2.5°C/sec |
| Thermal Cycler Block | 96-well |
| Heating Rate | 3.5°C/sec |
| Cooling Rate | 2.5°C/sec |
| Power Requirements | 100-240 V, 50-60 Hz |
