Applied Biosystems 7300 Getting Started Guide

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 59
Notes
Chapter 6
6
Analyzing Data in an RQ Study
Create a new
RQ Study document
Configure
analysis settings
Analyze and
view results
Export AQ
Plate document,
if desired
Adjust the
baseline and
threshold
If necessary,
omit samples
Performing
an RQ Study
D
es
i
gn
i
ng
an R
Q
E
xper
i
men
t
Performin
g
R
everse
Transcriptio
n
P
r
i
m
e
r
E
xt
e
n
ded
o
n m
RNA
5
´
3
´
Revers
e
Prime
r
5
´
Sy
nthesis of 1st cDNA strand
cDNA
O
ligo d(T) or random hexame
r
3
´
5
´
cDNA
OR
Generatin
g
Da
t
a
fr
om
RQ
Plate
s
S
tandard
STANDARD
TANDAR
G
eneratin
g
Da
t
a
fr
om
RQ
Plate
s
Fas
t
I
ntr
oduc
t
ion
and Exam
p
l
e
R
Q
Experiment
Seepage63
Seepage65
Seepage70
Seepage75
Seepage60
See page 78

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English