Applied Biosystems 7300 Getting Started Guide

Chapter 5 Generating Data from RQ Plates – 7500 Fast System
Preparing the Reaction Plate
40 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Notes
FAST
FAST
FAST
Preparing the Reaction Plate
Fast vs.
Standard Plates
IMPORTANT! Make sure that you use the Optical 96-Well Fast Plate on the 7500 Fast
System. Standard plates will not function properly and may be crushed when using the
96-Well Fast Block.
To Prepare the Reaction Plate:
1. Label the reaction plates, ensuring that you include an endogenous control for each
sample type (for example, each tissue in a study comparing multiple tissues). If
samples are spread across multiple plates, each plate must have an endogenous
control. Additionally, every plate must include an endogenous control for every
sample type on the plate.
2. Into each well of the reaction plate, add 20 µL into each well of the low head space
reaction plate of the appropriate PCR master mix.
3. Seal the reaction plate with an optical adhesive cover:
•ABIP
RISM
®
Optical Adhesive Cover, quantity 100 (PN 4311971)
• Optical Adhesive Cover, quantity 25 (PN 4360954)
4. Centrifuge the plate briefly.
5. Verify that each reaction is positioned in the bottom of the well.
GR2386
7900
HS96 well plate comparison
100-µL
maximum
reaction
volume
Fast Plates Standard Plates
Notch at top-right
corner by A12
30-µL
maximum
reaction
volume
Notch at
top-left
corner
by A1
(PN 4346906) d(PN 4306737)

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English