Chapter 5 Generating Data from RQ Plates – 7500 Fast System
Preparing the Reaction Plate
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 41
Notes
FAST
FAST
5
IMPORTANT! Ensure all reaction is positioned correctly in the bottom of the well before
starting a run. Failure to do so will impact the quality of data.
6. Place the reaction plates on ice until you are ready to load them into the 7500 Fast
system.
Correct Position Incorrect Positions
The reaction is positioned
correctly in the bottom of
the well.
The reaction lies on the
side wall because the
plate was not centrifuged.
An air bubble lies at the
bottom of the well
because the plate was not
centrifuged with sufficient
force or for sufficient time.
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