92 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Index
documents
exporting 36, 55, 78
RQ Plate 25, 43
RQ Study 60
templates 26, 43
dyes
FAM 14, 80
reporter 4
ROX 28, 35, 46, 54
SYBR Green I 11, 13
TAMRA 35, 54, 80
E
emulation mode, 9600 32
endogenous controls
associating with detectors 25, 43
definition 9
for RQ plates 25, 43
selecting for RQ studies 63
endpoint PCR 2
equipment 5
example RQ experiment 5
components 10
creating detectors 80
description 81
overview 5
PCR master mix 24, 42
PCR method 8
reverse transcription 20
RQ Plate document, example 29, 47
RQ Study document, example 70
Expert Mode 48, 50
exponential phase of amplification curve 65
exporting data
RQ plates 36, 55
RQ studies 78
F
FAM dye 14, 80
Fast system
creating an RQ plate document 43
example experiment 42
generating data from RQ plates 37
requirements 38
thermal cycling conditions 49
Fast vs. standard plates 40
G
Gene Expression plots 72
graph settings 33, 52, 71, 72
guidelines
preparing RNA 18
H
High Capacity cDNA Archive kit 19
I
importing plate setup information 26, 43
Information Development department, contacting ix
Instrument tab 32, 50
italic text, when to use vii
L
line width 33, 52, 71, 72
linear phase of amplification curve 65
M
master mix, PCR 22, 38
materials 5
menu commands, conventions for describing vii
mode, emulation 32
MSDSs, obtaining x
multiplex PCR 8
N
New Detector dialog box 79
normalized reporter 4
O
options, display 33, 52, 71, 72
options, graph 33, 52, 71, 72
outliers 75
P
passive reference 4, 28, 46
PCR
end-point 2
master mix, preparing 22, 38
multiplex 8
real-time 2
selecting a method 8
singleplex 8
starting an RQ plate run 32, 51
phases of amplification curve 65
Plate view 34, 53
plate, RQ. See RQ plates
plateau phase of amplification curve 65
plot appearance 33, 52, 71, 72
Primer Express Software 14
primers 14
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