Applied Biosystems 7300 Getting Started Guide

Chapter 5 Generating Data from RQ Plates – 7500 Fast System
Specifying Thermal Cycling Conditions and Starting the Run
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 49
Notes
FAST
FAST
FAST
5
Default Thermal
Cycling
Conditions for
PCR
If you selected the two-step RT-PCR method for your RQ experiment (recommended),
you have already completed the RT step and are ready to PCR amplify cDNA.
The default thermal cycling conditions for the PCR step of the procedure, shown in the
following table, should appear in the Instrument tab.
Fast Default Times and Temperatures (Two-step RT-PCR)
1) RT Step
HOLD HOLD * For reference only.
RT is complete at this point.
10 min @ 25 ° C 120 min @ 37 ° C
Fast Thermal Cycling Conditions (Fast 7500 users only)
2) PCR Step
Fast Conditions
Enzyme Activation Melt Anneal/Extend
95 ° C
0.20
3 sec @ 95 ° C 30 sec @ 60 ° C

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English