Applied Biosystems 7300 Getting Started Guide

Primer Extended on mRNA
5′
3′
Reverse
Primer
5′
Synthesis of 1st cDNA strand
cDNA
Oligo d(T) or random hexamer
3′
5′
cDNA
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 17
Notes
Chapter 3
3
Performing Reverse Transcription
Isolate total RNA
Adjust RNA
concentration
Convert
total RNA to cDNA
Performing
Reverse
Transcription
Intr
oduc
ti
on
and Exam
p
l
e
R
Q
Experimen
t
Desi
g
nin
g
an RQ
Experimen
t
P
r
i
m
e
r
E
xt
e
n
ded
o
n m
RNA
5
´
3
´
Revers
e
P
r
i
m
e
r
5
´
Sy
nthesis of 1st cDNA stran
d
c
DN
A
O
ligo d(T) or random hexame
r
3
´
5
´
cDNA
Performin
g
an R
Q
S
tudy
OR
Generatin
g
D
a
t
a
fr
o
m
R
Q
Plates
S
tandard
STANDARD
TANDAR
Generatin
g
Da
t
a
fr
om
RQ
Plate
s
Fas
t
Seepage18
Seepage18
See page 19

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English