Chapter 4 Generating Data from RQ Plates – 7300 or Standard 7500 System
Creating a Relative Quantification (RQ) Plate Document
28 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Notes
STANDARD
STANDARD
8. Enter the sample names.
a. In the Well Inspector, click a well or click-
drag to select replicate wells.
b. Enter the sample name.
c. If necessary, change the setting for the
Passive Reference dye. (By default, the
ROX
™
dye is selected.)
d. Repeat steps a through c until you specify
sample names and passive reference dyes
for all the wells on the plate.
IMPORTANT! If your experiment does not
use all the wells on a plate, do not omit the
wells from use at this point. You can omit
unused wells after the run. For information
about omitting unused wells, refer to the
Online Help.
Note: You can change the sample setup
information (sample name, detector, task)
after a run is complete, if necessary.
e. Close the Well Inspector.
9. Verify the information on each well in the Setup
tab.
8a
8b
8e 8c