Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 91
Index
Numerics
3D bars 72
5’-nuclease assay 11
9600 Emulation Mode 32
A
AIF. See assay information files
AmpErase UNG 12
amplification curve 65
amplification plots
Amplification Plot view for RQ plates 35, 54
types of amplification plots for RQ studies 70
appearance of graphs 33, 52, 71, 72
Applied Biosystems
contacting x
customer feedback on documentation ix
Information Development department ix
Technical Support x
assay information files 80
assumptions for using this guide vii
autoscaling options 33, 52, 71, 72
B
background in amplification curve 65
bar width 72
baseline
adjusting 65
definition 4
examples 66
bold text, when to use vii
C
calibrating the 7300/7500 instrument 22
calibrating the 7500 Fast instrument 38
calibrator
definition 9
selecting in RQ studies 63
CAUTION, description vii
cDNA
generating 19
storing 20
See also reverse transcription
Chemistries 11
comparative method of calculation 3, 60
Component view 35, 53
concentration of RNA 18
confidence levels 64
conventions
bold text vii
for describing menu commands vii
IMPORTANTS! vii
in this guide vii
italic text vii
Notes vii
user attention words vii
Ct. See threshold cycle
curves
amplification 65
standard 2
customer feedback, on Applied Biosystems
documents ix
D
data
analyzing 33, 52, 70
exporting 36, 55, 78
generating PCR data from RQ plates 32, 50
importing 26, 43
omitting from a study 75
delta Rn 4
Delta Rn vs.Cycle view 71
designing RQ experiments
determining reagent configuration 11
PCR method 8
primers and probes 14
selecting the chemistry 11
Detector Manager dialog box 79
detector tasks, definition 25, 43
detectors
adding to RQ plates 27, 45
creating 79
definition 79
selecting for RQ studies 63
deviation, standard 67
display options 33, 52, 71, 72
documentation, related ix
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