Applied Biosystems 7300 Getting Started Guide

Chapter 4 Generating Data from RQ Plates – 7300 or Standard 7500 System
Specifying Thermal Cycling Conditions and Starting the Run
32 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Notes
STANDARD
STANDARD
STANDARD
To specify thermal cycling conditions and start the
run:
1. Select the Instrument tab.
By default, the standard PCR conditions for the
PCR step of the two-step RT-PCR method are
displayed.
2. Verify that:
• For two-step RT-PCR, the default PCR
thermal cycling conditions are set.
• For one-step RT-PCR, you set the thermal
cycling parameters as shown above.
• Sample volume is 50 µL.
• 9600 Emulation is selected.
Note: If you are using SYBR
®
Green I reagent
chemistry and you want to determine if there is
contamination or if you want to determine the
dissociation temperature, create a separate
Dissociation assay or template. Refer to the
Online Help for more information.
Note: In the 7300 instrument, the 9600
Emulation feature is not available.
3. Select File > Save As, enter a name for the RQ
Plate document, then click Save.
4. Load the plate into the instrument.
Note: 7300 and 7500 standard plates are notched
in the A12 position at the top-right; Fast plates
are notched in the A1 position at the top-left.
5. Click Start.
As the instrument performs the PCR run, it
displays real-time status information in the
Instrument tab and records the fluorescence
emissions.
After the run, a message indicates whether or not
the run is successful.
All data generated during the run are saved to the
RQ Plate document that you saved in step 3.
GR2475
GR2475
7500
keyed corner
A1
A
B
C
D
E
F
G
H
123456789101112
A
1
Well A1
Keyed
corner for
standard
plates
12
Keyed corner for
Fast plates

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English