Chapter 4 Generating Data from RQ Plates – 7300 or Standard 7500 System
Analyzing and Viewing RQ Plate Data
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 35
Notes
STANDARD
STANDARD
4
Component Tab Displays the complete spectral contribution of each dye in a selected well over the
duration of the PCR run. Only the first selected well is shown at one time.
Note: If you are using TaqMan
®
products, three components (ROX
™
dye, reporter dye,
and TAMRA
™
dye-labelled quencher) are displayed in the Component tab. If you are
using TaqMan
®
MGB products, only two components (ROX and reporter dyes) are
displayed, as shown.
Amplification Plot
Tab
Displays a plot of R
n
as a function of cycle number for the selected detector and well(s).
Reanalyzing Data Raw fluorescence data (spectra), R
n
values, and well information (sample name,
detector, and detector task) are saved in an RQ plate document.
If you decide to omit wells or change well information after a run is complete, you must
reanalyze the data.
Note: After the software analyzes data, the Analyze button is disabled ( ). Whenever
you change a setting that requires reanalysis, the Analyze button is enabled ( ).
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