Applied Biosystems 7300 Getting Started Guide

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 93
Index
probes 14, 80
R
reagent configurations 12
Real-Time PCR assays 2
reference sample.See calibrator
reference, passive 4, 28, 46
relative quantification
comparative method of calculation 3
definition 2
experiments.See also RQ experiments 3
real-time PCR 2
references 2
RQ plates 3
RQ studies 3
replicates 9
reporter dye 4
Results tab 33, 52
results, RQ Study analysis 70
reverse transcription
guidelines for preparing RNA 18
High Capacity cDNA Archive kit 19
thermal cycling parameters 19
Rn vs. Cycle view 71
Rn. See normalized reporter
RNA
guidelines for preparing 18
isolation 18
starting concentration 18
ROX dye 28, 35, 46, 54
RQ Detector grid 64
RQ experiments
chemistries 11
components 9
probes and primers 14
reagent configuration 11
requirements 9
workflow 3
RQ Main Study view 62
RQ plates
adding to RQ studies 61
Amplification Plot view 35, 54
analyzing 33, 52
Component view 35, 53
data types 36, 55
definition 3
detectors, creating 79
exporting data 36, 55
Plate view 34, 53
reanalyzing data 35, 54
results 33, 52
RQ Plate documents 25, 43
Spectra view 34, 53
starting a run 32, 51
RQ Results panel 70
RQ Sample grid 70
RQ studies
adding RQ plates 61
Amplification Plots 70
confidence level 64
control type 64
data types 78
definition 3
exporting data 78
Gene Expression plots 72
omitting samples from 75
orientation 72
reanalyzing data 74
results 70
RQ Study documents 60
RT-PCR
one-step 12, 31
two-step 12, 30, 49
S
settings, graph 33, 52, 71, 72
Setup tab 28, 46
singleplex PCR 8
Spectra view 34, 53
standard curves 2
standard deviation, effect of threshold on 67
standard error 67
standard vs. Fast plates 23
starting an RQ plate run 32, 51
study, RQ. See RQ studies
SYBR Green I dye chemistry 11
T
TAMRA dye 35, 54, 80
TaqMan Assays 14
TaqMan Custom Gene Expression Assays 14
TaqMan MGB probes 15, 80
TaqMan reagent chemistry 11
TaqMan Universal PCR Master Mix 22
TaqMan® Gene Expression Assays 14
target
associating with detectors 25, 43
definition 9
tasks. See detector tasks
Technical Support, contacting x
template documents 26, 43
text conventions vii
thermal cycling conditions
default for PCR 30, 49

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English