94 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Index
one-step RT-PCR 31
specifying 32, 50
two-step RT-PCR 30, 49
thermal cycling parameters
for plates added to a study 61, 85
RT using High Capacity cDNA Archive kit 19
threshold
adjusting 65
definition 4
examples 67
threshold cycle
definition 4
setting for RQ studies 63
training, information on x
U
uracil-N-glycosylase 12
user attention words, described vii
W
WARNING, description vii
Well Information tab 70
wells, replicate 9
width, bars 72
width, lines 33, 52, 71, 72
workflow, RQ experiment overview 3
X
x-axis 33, 52, 71, 72
Y
y-axis 33, 52, 71, 72