Chapter 6 Analyzing Data in an RQ Study
Creating an RQ Study Document
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 61
Notes
6
To create a new RQ Study document:
1. Select File> New.
2. In the Assay drop-down list of the New
Document Wizard, select Relative
Quantification (ddCt) Study. Accept the default
settings for Container and Template
(96-Well Clear and Blank Document).
3. Enter a name in the Default Plate Name field, or
accept the default.
4. Click Next>.
5. Add RQ plates to the study.
a. Click Add Plates.
Note: You can add up to 10 RQ plates to an
RQ study.
b. Select the plate(s) that you want to add to
the study, then click Open.
o
The selected plates are displayed.
IMPORTANT! All plates added to a study
must have identical thermal cycling
parameters—the same number of steps,
cycles, sample volume, emulation mode.
The SDS Software 1.3.1 will reject a plate if
it detects any differences. (The first plate
added to the study serves as the reference
plate against which other plates are
compared.)