Applied Biosystems 7300 Getting Started Guide

Chapter 6 Analyzing Data in an RQ Study
Creating an RQ Study Document
62 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Notes
6. Click Finish. If desired, save the RQ Study
document when prompted.
The SDS Software 1.3.1 opens a new RQ Study
document and displays the RQ Study main view
with its three panes:
a. RQ Detector grid – Allows you to select
detectors to associate with the loaded study.
For each detector, Color, Detector name,
Threshold value, Auto Ct, and Baseline are
displayed.
Note: At this point, all the values in the
Threshold, Auto Ct, and Baseline columns
are set to the default values (0.200000,
Manual, and [6,15], respectively).
b. RQ Sample grid – Displays the samples
associated with the selected detector(s). The
Sample Grid displays numerical results of
RQ calculations and has two subtabs:
Sample Summary and Well Information.
c. RQ Results panel – Contains the three
results-based tabs: Plate (default),
Amplification Plot, and Gene Expression.
Note: You can save the RQ Study
document now, or wait until after specifying
analysis settings and analyzing the data.
6a
6b
6c

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English