Applied Biosystems 7300 Getting Started Guide

Chapter 2 Designing an RQ Experiment
Choosing the Probes and Primers
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 15
Notes
2
Sample Experiment
For the example experiment, primers and probes for all the genes being studied are obtained from Applied Biosystems
Taq M an
®
Gene Expression Assays. Each assay consists of two unlabeled PCR primers and a FAM
™
dye-labeled TaqMan
®
MGB probe, provided as a 20✕ assay mix.
In the example experiment, all target probes are labeled with FAM dye; the endogenous control is also labeled with FAM
dye.
The following table provides the gene symbol, gene name, and Applied Biosystems Assay ID number (provided on the Web
site) for five of the genes studied in the example experiment, plus the endogenous control.
Gene Symbol Gene Name Assay ID #
ACVR1 acrosomal vesicle protein I Hs00153836 m1
ACVR2 activin A receptor, type II Hs00155658_m1
CCR2 chemokine (C-C motif) receptor 2 Hs00174150_m1
CD3D CD3D antigen, delta polypeptide (TiT3 complex) Hs00174158_m1
FLT4 fms-related tyrosine kinase 4 Hs00176607_m1
GAPDH glyceraldehyde-3-phosphate dehydrogenase Hs99999905_m1

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English