Chapter 9: Cleaning/Shutdown Procedure 45
9
Cleaning/Shutdown Procedure
The BD FACSCalibur, BD LSR II, and BD LSRFortessa do not have a Fluidic
Shutdown cycle that automatically replaces BD FACS™ sheath solution with BD
FACS shutdown solution or DI water. These procedures are for the BD
FACSCalibur, BD LSR II, Special Order BD LSR II, and Special Order BD
LSRFortessa systems as they do not have a fluidic shutdown cycle built into the
software.
We recommend daily cleaning and maintenance of the sample coupler and tubing
to avoid clogs.
Required Kit Components
Cleaning Procedures
1 Fill a clean container with approximately 300 mL of deionized (DI) water.
2 Remove the sheath line from the HTS.
3 Connect the purge tubing to the HTS sheath port.
Item number Part number Description Qty
13 340088 HTS purge tubing 1