User’s Guide126
single-stained 65, 88
conventions
safety symbols 11
creating
analysis objects 93
global worksheets 89
statistics view 93
CS&T particles 123
cuvette flow cell 112
CV, troubleshooting 109
cytometer 17
configuration 44, 66
disconnect error 110
setup 64
starting 34
troubleshooting 102
D
DAPI, cleaning after using 49
data
analyzing 88, 93
gating 93
recording 88, 90
debris, excessive 108
delay, laser 112
detector arrays 29
detectors 31
digital data 17
Diva software See software
droplet containment system 27
E
electronics
troubleshooting 110
event rate
erratic 107
high 106
low 106
zero 103, 105
excessive debris 108
experiments
creating 72
immunophenotyping 89
sample optimization 72
specifying parameters 73
F
FACSFlow sheath fluid 123
FACSFlow solution 37
Falcon tubes 103
filters, optical
bandpass (BP) 30
filters, sheath
attaching new 57
replacing 56
fixed-alignment lasers 17
flow cell
draining 42
flow rate control buttons 23
fluid control buttons
PRIME 22
RUN 22
STANDBY 22
fluidics
description 21
flow rate control 23
flushing system 52
priming 42
removing air bubbles 38
run mode 22
sample injection port (SIP) 26
fluidics controls, cytometer 20
fluidics interconnect 39
fluorescence signal, none 105
fluorochromes
measured 44
flushing the system 52
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