54 | 405 TS Getting Started Guide
Aspirate Step Settings:
Z = 46 steps
X = 35
Y = 0
Secondary Aspirate:
Z = 80 steps
X = -45
Y = 0
About 40 µL/well residual fluid is
retained using these values.
For loosely adherent cells, the best performance was seen by increasing the aspirate
height and using both a standard and secondary (or crosswise) aspiration. Moving the
aspirate tubes from one side of the well to the other prevents a fluid stream from forming
and dislodging the cells. Increased residual in the well means increased cell retention.
Best practice:
l Use the Align tool to determine the optimal X, Y, and Z axis adjustments
needed to best position the manifold above the wells during the wash routine.
l Test the protocol settings by running the protocol using only water and an
empty plate before actually running your assay to make sure the fluid stream
hits the wells as desired.
Delay Aspiration or Vacuum-On Volume Control
Touch Screen Users: Edit the Wash or Dispense Step>Advanced Options
During regular plate washing, aspiration and dispensing occurs simultaneously.
This allows "overflow" dispensing, because the fluid is aspirated before
overflowing the plate. But, the low-flow tubing used in cell wash protocols
dispenses fluid so slowly that aspiration must be delayed to allow the fluid to
reach the well.
405™ TS Microplate Washer
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