82 | 405 TS Getting Started Guide
1. Run W-RINSE_AND_SOAK.
2. When the wash manifold is submerged, turn off the instrument.
5. Run a System Self-Test to empty the washer's priming trough when restarting
the instrument.
Removing Protein Residuals and Fungi Growth
Important! Solutions containing proteins, such as bovine serum albumin
(BSA), will compromise the 405 TS’s performance over time unless a strict
maintenance regime is adhered to. Do not use isopropyl alcohol to flush out
BSA.
When using protein solutions or similar fluids, BioTek recommends performing the
following additional Maintenance procedures to thoroughly flush out protein
particles and other contaminants from the fluid path.
Daily Practice with buffer or deionized water:
If the 405 TS will be idle between plates for longer than 45 minutes, flush the
proteins:
1. Fill a supply bottle with deionized water. Connect the bottle to the washer.
(Buffer valve “A” for Buffer Switching models)
2. Run the W-DAY_RINSE protocol.
3. Enable AutoPrime for 60-minute intervals.
At the end of the day:
1. Fill a supply bottle with deionized water. Connect the bottle to the washer
(Buffer valve “A” for Buffer Switching models).
2. Run the W-DAY_RINSE protocol three times.
3. Perform your regular Overnight/Multi-Day Maintenance routine.
Weekly or As Needed use NaOH and HCl to remove proteins:
1. Flush the system with 0.1-0.5 N* NaOH (sodium hydroxide), followed by
neutralization with an equivalent normality (0.1-0.5 N) of HCl (hydrochloride).
2. Rinse well with deionized water to remove the HCl.
3. Run the applicable DAY_RINSE protocol three times with deionized water if
you plan to use the device immediately.
405™ TS Microplate Washer
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